diff --git a/rnaseq/step5_count/snippet_counts b/rnaseq/step5_count/snippet_counts new file mode 100644 index 0000000..b2d791c --- /dev/null +++ b/rnaseq/step5_count/snippet_counts @@ -0,0 +1,18 @@ +## Generate Raw Counts using FeatureCounts/HTSeqCounts + +## Load package Subread (packge within which FeatureCounts is present) + ++ ml Subread/1.6.4-foss-2015b FeatureCounts + +## Features used in FeatureCounts : +## -s : reversely stranded +## -T : number of threads used +## -a : reference genome +## -o : output file + ++ featureCounts \ ++ --a "path to reference genome" \ ++ --T 6 \ ++ --o "path to output file" \ ++ --s 2 +