## Generate Raw Counts using FeatureCounts/HTSeqCounts ## Load package Subread (packge within which FeatureCounts is present) + ml Subread/1.6.4-foss-2015b FeatureCounts ## Features used in FeatureCounts : ## -s : reversely stranded ## -T : number of threads used ## -a : reference genome ## -o : output file + featureCounts \ + --a "path to reference genome" \ + --T 6 \ + --o "path to output file" \ + --s 2