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< h1 > How to work with WHONET data< / h1 >
< h4 class = "author" > Matthijs S. Berends< / h4 >
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< h4 class = "date" > 23 February 2020< / h4 >
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< div class = "hidden name" > < code > WHONET.Rmd< / code > < / div >
< / div >
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< div id = "import-of-data" class = "section level3" >
< h3 class = "hasAnchor" >
< a href = "#import-of-data" class = "anchor" > < / a > Import of data< / h3 >
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< p > This tutorial assumes you already imported the WHONET data with e.g. < a href = "https://readxl.tidyverse.org/" > < code > readxl< / code > package< / a > . In RStudio, this can be done using the menu button ‘ ’ ‘ ’ ‘ ’ < / p >
< p > An example syntax could look like this:< / p >
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< div class = "sourceCode" id = "cb1" > < pre class = "sourceCode r" > < code class = "sourceCode r" > < span id = "cb1-1" > < a href = "#cb1-1" > < / a > < span class = "kw" > < a href = "https://rdrr.io/r/base/library.html" > library< / a > < / span > (readxl)< / span >
< span id = "cb1-2" > < a href = "#cb1-2" > < / a > data < -< span class = "st" > < / span > < span class = "kw" > < a href = "https://readxl.tidyverse.org/reference/read_excel.html" > read_excel< / a > < / span > (< span class = "dt" > path =< / span > < span class = "st" > "path/to/your/file.xlsx"< / span > )< / span > < / code > < / pre > < / div >
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< p > This package comes with an < a href = "https://msberends.gitlab.io/AMR/reference/WHONET.html" > example data set < code > WHONET< / code > < / a > . We will use it for this analysis.< / p >
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< / div >
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< div id = "preparation" class = "section level3" >
< h3 class = "hasAnchor" >
< a href = "#preparation" class = "anchor" > < / a > Preparation< / h3 >
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< p > First, load the relevant packages if you did not yet did this. I use the tidyverse for all of my analyses. All of them. If you don’ < a href = "https://www.tidyverse.org/" class = "uri" > https://www.tidyverse.org/< / a > .< / p >
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< div class = "sourceCode" id = "cb2" > < pre class = "sourceCode r" > < code class = "sourceCode r" > < span id = "cb2-1" > < a href = "#cb2-1" > < / a > < span class = "kw" > < a href = "https://rdrr.io/r/base/library.html" > library< / a > < / span > (dplyr) < span class = "co" > # part of tidyverse< / span > < / span >
< span id = "cb2-2" > < a href = "#cb2-2" > < / a > < span class = "kw" > < a href = "https://rdrr.io/r/base/library.html" > library< / a > < / span > (ggplot2) < span class = "co" > # part of tidyverse< / span > < / span >
< span id = "cb2-3" > < a href = "#cb2-3" > < / a > < span class = "kw" > < a href = "https://rdrr.io/r/base/library.html" > library< / a > < / span > (AMR) < span class = "co" > # this package< / span > < / span > < / code > < / pre > < / div >
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< p > We will have to transform some variables to simplify and automate the analysis:< / p >
< ul >
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< li > Microorganisms should be transformed to our own microorganism IDs (called an < code > mo< / code > ) using < a href = "https://msberends.gitlab.io/AMR/reference/catalogue_of_life" > our Catalogue of Life reference data set< / a > , which contains all ~70,000 microorganisms from the taxonomic kingdoms Bacteria, Fungi and Protozoa. We do the tranformation with < code > < a href = "../reference/as.mo.html" > as.mo()< / a > < / code > . This function also recognises almost all WHONET abbreviations of microorganisms.< / li >
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< li > Antimicrobial results or interpretations have to be clean and valid. In other words, they should only contain values < code > "S"< / code > , < code > "I"< / code > or < code > "R"< / code > . That is exactly where the < code > < a href = "../reference/as.rsi.html" > as.rsi()< / a > < / code > function is for.< / li >
< / ul >
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< div class = "sourceCode" id = "cb3" > < pre class = "sourceCode r" > < code class = "sourceCode r" > < span id = "cb3-1" > < a href = "#cb3-1" > < / a > < span class = "co" > # transform variables< / span > < / span >
< span id = "cb3-2" > < a href = "#cb3-2" > < / a > data < -< span class = "st" > < / span > WHONET < span class = "op" > %> %< / span > < / span >
< span id = "cb3-3" > < a href = "#cb3-3" > < / a > < span class = "st" > < / span > < span class = "co" > # get microbial ID based on given organism< / span > < / span >
< span id = "cb3-4" > < a href = "#cb3-4" > < / a > < span class = "st" > < / span > < span class = "kw" > < a href = "https://dplyr.tidyverse.org/reference/mutate.html" > mutate< / a > < / span > (< span class = "dt" > mo =< / span > < span class = "kw" > < a href = "../reference/as.mo.html" > as.mo< / a > < / span > (Organism)) < span class = "op" > %> %< / span > < span class = "st" > < / span > < / span >
< span id = "cb3-5" > < a href = "#cb3-5" > < / a > < span class = "st" > < / span > < span class = "co" > # transform everything from "AMP_ND10" to "CIP_EE" to the new `rsi` class< / span > < / span >
< span id = "cb3-6" > < a href = "#cb3-6" > < / a > < span class = "st" > < / span > < span class = "kw" > < a href = "https://dplyr.tidyverse.org/reference/mutate_all.html" > mutate_at< / a > < / span > (< span class = "kw" > < a href = "https://dplyr.tidyverse.org/reference/vars.html" > vars< / a > < / span > (AMP_ND10< span class = "op" > :< / span > CIP_EE), as.rsi)< / span > < / code > < / pre > < / div >
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< p > No errors or warnings, so all values are transformed succesfully.< / p >
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< p > We also created a package dedicated to data cleaning and checking, called the < code > cleaner< / code > package. It gets automatically installed with the < code > AMR< / code > package. For its < code > < a href = "https://rdrr.io/pkg/cleaner/man/freq.html" > freq()< / a > < / code > function to create frequency tables, you don’ < code > AMR< / code > package as well.< / p >
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< p > So let’ < / p >
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< div class = "sourceCode" id = "cb4" > < pre class = "sourceCode r" > < code class = "sourceCode r" > < span id = "cb4-1" > < a href = "#cb4-1" > < / a > < span class = "co" > # our newly created `mo` variable, put in the mo_name() function< / span > < / span >
< span id = "cb4-2" > < a href = "#cb4-2" > < / a > data < span class = "op" > %> %< / span > < span class = "st" > < / span > < span class = "kw" > < a href = "https://rdrr.io/pkg/cleaner/man/freq.html" > freq< / a > < / span > (< span class = "kw" > < a href = "../reference/mo_property.html" > mo_name< / a > < / span > (mo), < span class = "dt" > nmax =< / span > < span class = "dv" > 10< / span > )< / span > < / code > < / pre > < / div >
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< p > < strong > Frequency table< / strong > < / p >
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< p > Class: character< br >
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Length: 500< br >
Available: 500 (100%, NA: 0 = 0%)< br >
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Unique: 39< / p >
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< p > Shortest: 11< br >
Longest: 40< / p >
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< table class = "table" >
< thead > < tr class = "header" >
< th align = "left" > < / th >
< th align = "left" > Item< / th >
< th align = "right" > Count< / th >
< th align = "right" > Percent< / th >
< th align = "right" > Cum. Count< / th >
< th align = "right" > Cum. Percent< / th >
< / tr > < / thead >
< tbody >
< tr class = "odd" >
< td align = "left" > 1< / td >
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< td align = "left" > Escherichia coli< / td >
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< td align = "right" > 245< / td >
< td align = "right" > 49.0%< / td >
< td align = "right" > 245< / td >
< td align = "right" > 49.0%< / td >
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< / tr >
< tr class = "even" >
< td align = "left" > 2< / td >
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< td align = "left" > Coagulase-negative Staphylococcus (CoNS)< / td >
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< td align = "right" > 74< / td >
< td align = "right" > 14.8%< / td >
< td align = "right" > 319< / td >
< td align = "right" > 63.8%< / td >
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< / tr >
< tr class = "odd" >
< td align = "left" > 3< / td >
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< td align = "left" > Staphylococcus epidermidis< / td >
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< td align = "right" > 38< / td >
< td align = "right" > 7.6%< / td >
< td align = "right" > 357< / td >
< td align = "right" > 71.4%< / td >
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< / tr >
< tr class = "even" >
< td align = "left" > 4< / td >
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< td align = "left" > Streptococcus pneumoniae< / td >
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< td align = "right" > 31< / td >
< td align = "right" > 6.2%< / td >
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< td align = "right" > 388< / td >
< td align = "right" > 77.6%< / td >
< / tr >
< tr class = "odd" >
< td align = "left" > 5< / td >
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< td align = "left" > Staphylococcus hominis< / td >
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< td align = "right" > 21< / td >
< td align = "right" > 4.2%< / td >
< td align = "right" > 409< / td >
< td align = "right" > 81.8%< / td >
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< / tr >
< tr class = "even" >
< td align = "left" > 6< / td >
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< td align = "left" > Proteus mirabilis< / td >
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< td align = "right" > 9< / td >
< td align = "right" > 1.8%< / td >
< td align = "right" > 418< / td >
< td align = "right" > 83.6%< / td >
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< / tr >
< tr class = "odd" >
< td align = "left" > 7< / td >
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< td align = "left" > Enterococcus faecium< / td >
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< td align = "right" > 8< / td >
< td align = "right" > 1.6%< / td >
< td align = "right" > 426< / td >
< td align = "right" > 85.2%< / td >
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< / tr >
< tr class = "even" >
< td align = "left" > 8< / td >
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< td align = "left" > Staphylococcus capitis< / td >
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< td align = "right" > 8< / td >
< td align = "right" > 1.6%< / td >
< td align = "right" > 434< / td >
< td align = "right" > 86.8%< / td >
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< / tr >
< tr class = "odd" >
< td align = "left" > 9< / td >
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< td align = "left" > Enterobacter cloacae< / td >
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< td align = "right" > 5< / td >
< td align = "right" > 1.0%< / td >
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< td align = "right" > 439< / td >
< td align = "right" > 87.8%< / td >
< / tr >
< tr class = "even" >
< td align = "left" > 10< / td >
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< td align = "left" > Enterococcus columbae< / td >
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< td align = "right" > 4< / td >
< td align = "right" > 0.8%< / td >
< td align = "right" > 443< / td >
< td align = "right" > 88.6%< / td >
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< / tr >
< / tbody >
< / table >
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< p > (omitted 29 entries, n = 57 [11.40%])< / p >
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< div class = "sourceCode" id = "cb5" > < pre class = "sourceCode r" > < code class = "sourceCode r" > < span id = "cb5-1" > < a href = "#cb5-1" > < / a > < span class = "co" > # our transformed antibiotic columns< / span > < / span >
< span id = "cb5-2" > < a href = "#cb5-2" > < / a > < span class = "co" > # amoxicillin/clavulanic acid (J01CR02) as an example< / span > < / span >
< span id = "cb5-3" > < a href = "#cb5-3" > < / a > data < span class = "op" > %> %< / span > < span class = "st" > < / span > < span class = "kw" > < a href = "https://rdrr.io/pkg/cleaner/man/freq.html" > freq< / a > < / span > (AMC_ND2)< / span > < / code > < / pre > < / div >
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< p > < strong > Frequency table< / strong > < / p >
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< p > Class: factor > ordered > rsi (numeric)< br >
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Length: 500< br >
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Levels: 3: S < I < R< br >
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Available: 481 (96.2%, NA: 19 = 3.8%)< br >
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Unique: 3< / p >
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< p > %SI: 78.6%< / p >
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< table class = "table" >
< thead > < tr class = "header" >
< th align = "left" > < / th >
< th align = "left" > Item< / th >
< th align = "right" > Count< / th >
< th align = "right" > Percent< / th >
< th align = "right" > Cum. Count< / th >
< th align = "right" > Cum. Percent< / th >
< / tr > < / thead >
< tbody >
< tr class = "odd" >
< td align = "left" > 1< / td >
< td align = "left" > S< / td >
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< td align = "right" > 356< / td >
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< td align = "right" > 74.01%< / td >
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< td align = "right" > 356< / td >
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< td align = "right" > 74.01%< / td >
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< / tr >
< tr class = "even" >
< td align = "left" > 2< / td >
< td align = "left" > R< / td >
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< td align = "right" > 103< / td >
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< td align = "right" > 21.41%< / td >
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< td align = "right" > 459< / td >
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< td align = "right" > 95.43%< / td >
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< / tr >
< tr class = "odd" >
< td align = "left" > 3< / td >
< td align = "left" > I< / td >
< td align = "right" > 22< / td >
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< td align = "right" > 4.57%< / td >
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< td align = "right" > 481< / td >
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< td align = "right" > 100.00%< / td >
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< / tr >
< / tbody >
< / table >
< / div >
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< div id = "a-first-glimpse-at-results" class = "section level3" >
< h3 class = "hasAnchor" >
< a href = "#a-first-glimpse-at-results" class = "anchor" > < / a > A first glimpse at results< / h3 >
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< p > An easy < code > ggplot< / code > will already give a lot of information, using the included < code > < a href = "../reference/ggplot_rsi.html" > ggplot_rsi()< / a > < / code > function:< / p >
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< div class = "sourceCode" id = "cb6" > < pre class = "sourceCode r" > < code class = "sourceCode r" > < span id = "cb6-1" > < a href = "#cb6-1" > < / a > data < span class = "op" > %> %< / span > < / span >
< span id = "cb6-2" > < a href = "#cb6-2" > < / a > < span class = "st" > < / span > < span class = "kw" > < a href = "https://dplyr.tidyverse.org/reference/group_by.html" > group_by< / a > < / span > (Country) < span class = "op" > %> %< / span > < / span >
< span id = "cb6-3" > < a href = "#cb6-3" > < / a > < span class = "st" > < / span > < span class = "kw" > < a href = "https://dplyr.tidyverse.org/reference/select.html" > select< / a > < / span > (Country, AMP_ND2, AMC_ED20, CAZ_ED10, CIP_ED5) < span class = "op" > %> %< / span > < / span >
< span id = "cb6-4" > < a href = "#cb6-4" > < / a > < span class = "st" > < / span > < span class = "kw" > < a href = "../reference/ggplot_rsi.html" > ggplot_rsi< / a > < / span > (< span class = "dt" > translate_ab =< / span > < span class = "st" > 'ab'< / span > , < span class = "dt" > facet =< / span > < span class = "st" > "Country"< / span > , < span class = "dt" > datalabels =< / span > < span class = "ot" > FALSE< / span > )< / span > < / code > < / pre > < / div >
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< p > Developed by < a href = "https://www.rug.nl/staff/m.s.berends/" > Matthijs S. Berends< / a > , < a href = "https://www.rug.nl/staff/c.f.luz/" > Christian F. Luz< / a > , < a href = "https://www.rug.nl/staff/a.w.friedrich/" > Alexander W. Friedrich< / a > , < a href = "https://www.rug.nl/staff/b.sinha/" > Bhanu N. M. Sinha< / a > , < a href = "https://www.rug.nl/staff/c.j.albers/" > Casper J. Albers< / a > , < a href = "https://www.rug.nl/staff/c.glasner/" > Corinna Glasner< / a > .< / p >
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