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# ==================================================================== #
# TITLE #
# Antimicrobial Resistance (AMR) Data Analysis for R #
# #
# SOURCE #
# https://github.com/msberends/AMR #
# #
# LICENCE #
# (c) 2018-2021 Berends MS, Luz CF et al. #
# Developed at the University of Groningen, the Netherlands, in #
# collaboration with non-profit organisations Certe Medical #
# Diagnostics & Advice, and University Medical Center Groningen. #
# #
# This R package is free software; you can freely use and distribute #
# it for both personal and commercial purposes under the terms of the #
# GNU General Public License version 2.0 (GNU GPL-2), as published by #
# the Free Software Foundation. #
# We created this package for both routine data analysis and academic #
# research and it was publicly released in the hope that it will be #
# useful, but it comes WITHOUT ANY WARRANTY OR LIABILITY. #
# #
# Visit our website for the full manual and a complete tutorial about #
# how to conduct AMR data analysis: https://msberends.github.io/AMR/ #
# ==================================================================== #
#' AMR Plots with `ggplot2`
#'
#' Use these functions to create bar plots for AMR data analysis. All functions rely on [ggplot2][ggplot2::ggplot()] functions.
#' @inheritSection lifecycle Stable Lifecycle
#' @param data a [data.frame] with column(s) of class [`rsi`] (see [as.rsi()])
#' @param position position adjustment of bars, either `"fill"`, `"stack"` or `"dodge"`
#' @param x variable to show on x axis, either `"antibiotic"` (default) or `"interpretation"` or a grouping variable
#' @param fill variable to categorise using the plots legend, either `"antibiotic"` (default) or `"interpretation"` or a grouping variable
#' @param breaks a [numeric] vector of positions
#' @param limits a [numeric] vector of length two providing limits of the scale, use `NA` to refer to the existing minimum or maximum
#' @param facet variable to split plots by, either `"interpretation"` (default) or `"antibiotic"` or a grouping variable
#' @inheritParams proportion
#' @param nrow (when using `facet`) number of rows
#' @param colours a named vactor with colour to be used for filling. The default colours are colour-blind friendly.
#' @param aesthetics aesthetics to apply the colours to, defaults to "fill" but can also be (a combination of) "alpha", "colour", "fill", "linetype", "shape" or "size"
#' @param datalabels show datalabels using [labels_rsi_count()]
#' @param datalabels.size size of the datalabels
#' @param datalabels.colour colour of the datalabels
#' @param title text to show as title of the plot
#' @param subtitle text to show as subtitle of the plot
#' @param caption text to show as caption of the plot
#' @param x.title text to show as x axis description
#' @param y.title text to show as y axis description
#' @param ... other arguments passed on to [geom_rsi()] or, in case of [scale_rsi_colours()], named values to set colours. The default colours are colour-blind friendly, while maintaining the convention that e.g. 'susceptible' should be green and 'resistant' should be red. See *Examples*.
#' @details At default, the names of antibiotics will be shown on the plots using [ab_name()]. This can be set with the `translate_ab` argument. See [count_df()].
#'
#' ## The Functions
#' [geom_rsi()] will take any variable from the data that has an [`rsi`] class (created with [as.rsi()]) using [rsi_df()] and will plot bars with the percentage R, I and S. The default behaviour is to have the bars stacked and to have the different antibiotics on the x axis.
#'
#' [facet_rsi()] creates 2d plots (at default based on S/I/R) using [ggplot2::facet_wrap()].
#'
#' [scale_y_percent()] transforms the y axis to a 0 to 100% range using [ggplot2::scale_y_continuous()].
#'
#' [scale_rsi_colours()] sets colours to the bars (green for S, yellow for I, and red for R). with multilingual support. The default colours are colour-blind friendly, while maintaining the convention that e.g. 'susceptible' should be green and 'resistant' should be red.
#'
#' [theme_rsi()] is a [ggplot2 theme][[ggplot2::theme()] with minimal distraction.
#'
#' [labels_rsi_count()] print datalabels on the bars with percentage and amount of isolates using [ggplot2::geom_text()].
#'
#' [ggplot_rsi()] is a wrapper around all above functions that uses data as first input. This makes it possible to use this function after a pipe (`%>%`). See *Examples*.
#' @rdname ggplot_rsi
#' @export
#' @inheritSection AMR Read more on Our Website!
#' @examples
#' \donttest{
#' if (require("ggplot2") & require("dplyr")) {
#'
#' # get antimicrobial results for drugs against a UTI:
#' ggplot(example_isolates %>% select(AMX, NIT, FOS, TMP, CIP)) +
#' geom_rsi()
#'
#' # prettify the plot using some additional functions:
#' df <- example_isolates %>% select(AMX, NIT, FOS, TMP, CIP)
#' ggplot(df) +
#' geom_rsi() +
#' scale_y_percent() +
#' scale_rsi_colours() +
#' labels_rsi_count() +
#' theme_rsi()
#'
#' # or better yet, simplify this using the wrapper function - a single command:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi()
#'
#' # get only proportions and no counts:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi(datalabels = FALSE)
#'
#' # add other ggplot2 arguments as you like:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi(width = 0.5,
#' colour = "black",
#' size = 1,
#' linetype = 2,
#' alpha = 0.25)
#'
#' # you can alter the colours with colour names:
#' example_isolates %>%
#' select(AMX) %>%
#' ggplot_rsi(colours = c(SI = "yellow"))
#'
#' # but you can also use the built-in colour-blind friendly colours for
#' # your plots, where "S" is green, "I" is yellow and "R" is red:
#' data.frame(x = c("Value1", "Value2", "Value3"),
#' y = c(1, 2, 3),
#' z = c("Value4", "Value5", "Value6")) %>%
#' ggplot() +
#' geom_col(aes(x = x, y = y, fill = z)) +
#' scale_rsi_colours(Value4 = "S", Value5 = "I", Value6 = "R")
#'
#' # resistance of ciprofloxacine per age group
#' example_isolates %>%
#' mutate(first_isolate = first_isolate()) %>%
#' filter(first_isolate == TRUE,
#' mo == as.mo("E. coli")) %>%
#' # age_groups() is also a function in this AMR package:
#' group_by(age_group = age_groups(age)) %>%
#' select(age_group,
#' CIP) %>%
#' ggplot_rsi(x = "age_group")
#'
#' # a shorter version which also adjusts data label colours:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi(colours = FALSE)
#'
#'
#' # it also supports groups (don't forget to use the group var on `x` or `facet`):
#' example_isolates %>%
#' select(hospital_id, AMX, NIT, FOS, TMP, CIP) %>%
#' group_by(hospital_id) %>%
#' ggplot_rsi(x = "hospital_id",
#' facet = "antibiotic",
#' nrow = 1,
#' title = "AMR of Anti-UTI Drugs Per Hospital",
#' x.title = "Hospital",
#' datalabels = FALSE)
#' }
#' }
ggplot_rsi <- function(data,
position = NULL,
x = "antibiotic",
fill = "interpretation",
# params = list(),
facet = NULL,
breaks = seq(0, 1, 0.1),
limits = NULL,
translate_ab = "name",
combine_SI = TRUE,
combine_IR = FALSE,
minimum = 30,
language = get_locale(),
nrow = NULL,
colours = c(S = "#3CAEA3",
SI = "#3CAEA3",
I = "#F6D55C",
IR = "#ED553B",
R = "#ED553B"),
datalabels = TRUE,
datalabels.size = 2.5,
datalabels.colour = "grey15",
title = NULL,
subtitle = NULL,
caption = NULL,
x.title = "Antimicrobial",
y.title = "Proportion",
...) {
stop_ifnot_installed("ggplot2")
meet_criteria(data, allow_class = "data.frame", contains_column_class = "rsi")
meet_criteria(position, allow_class = "character", has_length = 1, is_in = c("fill", "stack", "dodge"), allow_NULL = TRUE)
meet_criteria(x, allow_class = "character", has_length = 1)
meet_criteria(fill, allow_class = "character", has_length = 1)
meet_criteria(facet, allow_class = "character", has_length = 1, allow_NULL = TRUE)
meet_criteria(breaks, allow_class = c("numeric", "integer"))
meet_criteria(limits, allow_class = c("numeric", "integer"), has_length = 2, allow_NULL = TRUE, allow_NA = TRUE)
meet_criteria(translate_ab, allow_class = c("character", "logical"), has_length = 1, allow_NA = TRUE)
meet_criteria(combine_SI, allow_class = "logical", has_length = 1)
meet_criteria(combine_IR, allow_class = "logical", has_length = 1)
meet_criteria(minimum, allow_class = c("numeric", "integer"), has_length = 1, is_finite = TRUE)
meet_criteria(language, has_length = 1, is_in = c(LANGUAGES_SUPPORTED, ""), allow_NULL = TRUE, allow_NA = TRUE)
meet_criteria(nrow, allow_class = c("numeric", "integer"), has_length = 1, allow_NULL = TRUE, is_positive = TRUE, is_finite = TRUE)
meet_criteria(colours, allow_class = c("character", "logical"))
meet_criteria(datalabels, allow_class = "logical", has_length = 1)
meet_criteria(datalabels.size, allow_class = c("numeric", "integer"), has_length = 1, is_positive = TRUE, is_finite = TRUE)
meet_criteria(datalabels.colour, allow_class = "character", has_length = 1)
meet_criteria(title, allow_class = "character", has_length = 1, allow_NULL = TRUE)
meet_criteria(subtitle, allow_class = "character", has_length = 1, allow_NULL = TRUE)
meet_criteria(caption, allow_class = "character", has_length = 1, allow_NULL = TRUE)
meet_criteria(x.title, allow_class = "character", has_length = 1, allow_NULL = TRUE)
meet_criteria(y.title, allow_class = "character", has_length = 1, allow_NULL = TRUE)
# we work with aes_string later on
x_deparse <- deparse(substitute(x))
if (x_deparse != "x") {
x <- x_deparse
}
if (x %like% '".*"') {
x <- substr(x, 2, nchar(x) - 1)
}
facet_deparse <- deparse(substitute(facet))
if (facet_deparse != "facet") {
facet <- facet_deparse
}
if (facet %like% '".*"') {
facet <- substr(facet, 2, nchar(facet) - 1)
}
if (facet %in% c("NULL", "")) {
facet <- NULL
}
if (is.null(position)) {
position <- "fill"
}
p <- ggplot2::ggplot(data = data) +
geom_rsi(position = position, x = x, fill = fill, translate_ab = translate_ab,
minimum = minimum, language = language,
combine_SI = combine_SI, combine_IR = combine_IR, ...) +
theme_rsi()
if (fill == "interpretation") {
p <- p + scale_rsi_colours(colours = colours)
}
if (identical(position, "fill")) {
# proportions, so use y scale with percentage
p <- p + scale_y_percent(breaks = breaks, limits = limits)
}
if (datalabels == TRUE) {
p <- p + labels_rsi_count(position = position,
x = x,
translate_ab = translate_ab,
minimum = minimum,
language = language,
combine_SI = combine_SI,
combine_IR = combine_IR,
datalabels.size = datalabels.size,
datalabels.colour = datalabels.colour)
}
if (!is.null(facet)) {
p <- p + facet_rsi(facet = facet, nrow = nrow)
}
p <- p + ggplot2::labs(title = title,
subtitle = subtitle,
caption = caption,
x = x.title,
y = y.title)
p
}
#' @rdname ggplot_rsi
#' @export
geom_rsi <- function(position = NULL,
x = c("antibiotic", "interpretation"),
fill = "interpretation",
translate_ab = "name",
minimum = 30,
language = get_locale(),
combine_SI = TRUE,
combine_IR = FALSE,
...) {
x <- x[1]
stop_ifnot_installed("ggplot2")
stop_if(is.data.frame(position), "`position` is invalid. Did you accidentally use '%>%' instead of '+'?")
meet_criteria(position, allow_class = "character", has_length = 1, is_in = c("fill", "stack", "dodge"), allow_NULL = TRUE)
meet_criteria(x, allow_class = "character", has_length = 1)
meet_criteria(fill, allow_class = "character", has_length = 1)
meet_criteria(translate_ab, allow_class = c("character", "logical"), has_length = 1, allow_NA = TRUE)
meet_criteria(minimum, allow_class = c("numeric", "integer"), has_length = 1, is_finite = TRUE)
meet_criteria(language, has_length = 1, is_in = c(LANGUAGES_SUPPORTED, ""), allow_NULL = TRUE, allow_NA = TRUE)
meet_criteria(combine_SI, allow_class = "logical", has_length = 1)
meet_criteria(combine_IR, allow_class = "logical", has_length = 1)
y <- "value"
if (missing(position) | is.null(position)) {
position <- "fill"
}
if (identical(position, "fill")) {
position <- ggplot2::position_fill(vjust = 0.5, reverse = TRUE)
}
# we work with aes_string later on
x_deparse <- deparse(substitute(x))
if (x_deparse != "x") {
x <- x_deparse
}
if (x %like% '".*"') {
x <- substr(x, 2, nchar(x) - 1)
}
if (tolower(x) %in% tolower(c("ab", "abx", "antibiotics"))) {
x <- "antibiotic"
} else if (tolower(x) %in% tolower(c("SIR", "RSI", "interpretations", "result"))) {
x <- "interpretation"
}
ggplot2::geom_col(
data = function(x) {
rsi_df(data = x,
translate_ab = translate_ab,
language = language,
minimum = minimum,
combine_SI = combine_SI,
combine_IR = combine_IR)
},
mapping = ggplot2::aes_string(x = x, y = y, fill = fill),
position = position,
...
)
}
#' @rdname ggplot_rsi
#' @export
facet_rsi <- function(facet = c("interpretation", "antibiotic"), nrow = NULL) {
facet <- facet[1]
stop_ifnot_installed("ggplot2")
meet_criteria(facet, allow_class = "character", has_length = 1)
meet_criteria(nrow, allow_class = c("numeric", "integer"), has_length = 1, allow_NULL = TRUE, is_positive = TRUE, is_finite = TRUE)
# we work with aes_string later on
facet_deparse <- deparse(substitute(facet))
if (facet_deparse != "facet") {
facet <- facet_deparse
}
if (facet %like% '".*"') {
facet <- substr(facet, 2, nchar(facet) - 1)
}
if (tolower(facet) %in% tolower(c("SIR", "RSI", "interpretations", "result"))) {
facet <- "interpretation"
} else if (tolower(facet) %in% tolower(c("ab", "abx", "antibiotics"))) {
facet <- "antibiotic"
}
ggplot2::facet_wrap(facets = facet, scales = "free_x", nrow = nrow)
}
#' @rdname ggplot_rsi
#' @export
scale_y_percent <- function(breaks = seq(0, 1, 0.1), limits = NULL) {
stop_ifnot_installed("ggplot2")
meet_criteria(breaks, allow_class = c("numeric", "integer"))
meet_criteria(limits, allow_class = c("numeric", "integer"), has_length = 2, allow_NULL = TRUE, allow_NA = TRUE)
if (all(breaks[breaks != 0] > 1)) {
breaks <- breaks / 100
}
ggplot2::scale_y_continuous(breaks = breaks,
labels = percentage(breaks),
limits = limits)
}
#' @rdname ggplot_rsi
#' @export
scale_rsi_colours <- function(...,
aesthetics = "fill") {
stop_ifnot_installed("ggplot2")
meet_criteria(aesthetics, allow_class = "character", is_in = c("alpha", "colour", "color", "fill", "linetype", "shape", "size"))
# behaviour until AMR pkg v1.5.0 and also when coming from ggplot_rsi()
if ("colours" %in% names(list(...))) {
original_cols <- c(S = "#3CAEA3",
SI = "#3CAEA3",
I = "#F6D55C",
IR = "#ED553B",
R = "#ED553B")
colours <- replace(original_cols, names(list(...)$colours), list(...)$colours)
# limits = force is needed in ggplot2 3.3.4 and 3.3.5, see here;
# https://github.com/tidyverse/ggplot2/issues/4511#issuecomment-866185530
return(ggplot2::scale_fill_manual(values = colours, limits = force))
}
if (identical(unlist(list(...)), FALSE)) {
return(invisible())
}
names_susceptible <- c("S", "SI", "IS", "S+I", "I+S", "susceptible", "Susceptible",
unique(TRANSLATIONS[which(TRANSLATIONS$pattern == "Susceptible"),
"replacement", drop = TRUE]))
names_incr_exposure <- c("I", "intermediate", "increased exposure", "incr. exposure", "Increased exposure", "Incr. exposure",
unique(TRANSLATIONS[which(TRANSLATIONS$pattern == "Intermediate"),
"replacement", drop = TRUE]),
unique(TRANSLATIONS[which(TRANSLATIONS$pattern == "Incr. exposure"),
"replacement", drop = TRUE]))
names_resistant <- c("R", "IR", "RI", "R+I", "I+R", "resistant", "Resistant",
unique(TRANSLATIONS[which(TRANSLATIONS$pattern == "Resistant"),
"replacement", drop = TRUE]))
susceptible <- rep("#3CAEA3", length(names_susceptible))
names(susceptible) <- names_susceptible
incr_exposure <- rep("#F6D55C", length(names_incr_exposure))
names(incr_exposure) <- names_incr_exposure
resistant <- rep("#ED553B", length(names_resistant))
names(resistant) <- names_resistant
original_cols = c(susceptible, incr_exposure, resistant)
dots <- c(...)
# replace S, I, R as colours: scale_rsi_colours(mydatavalue = "S")
dots[dots == "S"] <- "#3CAEA3"
dots[dots == "I"] <- "#F6D55C"
dots[dots == "R"] <- "#ED553B"
cols <- replace(original_cols, names(dots), dots)
# limits = force is needed in ggplot2 3.3.4 and 3.3.5, see here;
# https://github.com/tidyverse/ggplot2/issues/4511#issuecomment-866185530
ggplot2::scale_discrete_manual(aesthetics = aesthetics, values = cols, limits = force)
}
#' @rdname ggplot_rsi
#' @export
theme_rsi <- function() {
stop_ifnot_installed("ggplot2")
ggplot2::theme_minimal(base_size = 10) +
ggplot2::theme(panel.grid.major.x = ggplot2::element_blank(),
panel.grid.minor = ggplot2::element_blank(),
panel.grid.major.y = ggplot2::element_line(colour = "grey75"),
# center title and subtitle
plot.title = ggplot2::element_text(hjust = 0.5),
plot.subtitle = ggplot2::element_text(hjust = 0.5))
}
#' @rdname ggplot_rsi
#' @export
labels_rsi_count <- function(position = NULL,
x = "antibiotic",
translate_ab = "name",
minimum = 30,
language = get_locale(),
combine_SI = TRUE,
combine_IR = FALSE,
datalabels.size = 3,
datalabels.colour = "grey15") {
stop_ifnot_installed("ggplot2")
meet_criteria(position, allow_class = "character", has_length = 1, is_in = c("fill", "stack", "dodge"), allow_NULL = TRUE)
meet_criteria(x, allow_class = "character", has_length = 1)
meet_criteria(translate_ab, allow_class = c("character", "logical"), has_length = 1, allow_NA = TRUE)
meet_criteria(minimum, allow_class = c("numeric", "integer"), has_length = 1, is_finite = TRUE)
meet_criteria(language, has_length = 1, is_in = c(LANGUAGES_SUPPORTED, ""), allow_NULL = TRUE, allow_NA = TRUE)
meet_criteria(combine_SI, allow_class = "logical", has_length = 1)
meet_criteria(combine_IR, allow_class = "logical", has_length = 1)
meet_criteria(datalabels.size, allow_class = c("numeric", "integer"), has_length = 1, is_positive = TRUE, is_finite = TRUE)
meet_criteria(datalabels.colour, allow_class = "character", has_length = 1)
if (is.null(position)) {
position <- "fill"
}
if (identical(position, "fill")) {
position <- ggplot2::position_fill(vjust = 0.5, reverse = TRUE)
}
x_name <- x
ggplot2::geom_text(mapping = ggplot2::aes_string(label = "lbl",
x = x,
y = "value"),
position = position,
inherit.aes = FALSE,
size = datalabels.size,
colour = datalabels.colour,
lineheight = 0.75,
data = function(x) {
transformed <- rsi_df(data = x,
translate_ab = translate_ab,
combine_SI = combine_SI,
combine_IR = combine_IR,
minimum = minimum,
language = language)
transformed$gr <- transformed[, x_name, drop = TRUE]
transformed %pm>%
pm_group_by(gr) %pm>%
pm_mutate(lbl = paste0("n=", isolates)) %pm>%
pm_ungroup() %pm>%
pm_select(-gr)
})
}
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