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# ==================================================================== #
# TITLE #
# Antimicrobial Resistance (AMR) Analysis #
# #
# SOURCE #
# https://gitlab.com/msberends/AMR #
# #
# LICENCE #
# (c) 2018-2020 Berends MS, Luz CF et al. #
# #
# This R package is free software; you can freely use and distribute #
# it for both personal and commercial purposes under the terms of the #
# GNU General Public License version 2.0 (GNU GPL-2), as published by #
# the Free Software Foundation. #
# #
# We created this package for both routine data analysis and academic #
# research and it was publicly released in the hope that it will be #
# useful, but it comes WITHOUT ANY WARRANTY OR LIABILITY. #
# Visit our website for more info: https://msberends.gitlab.io/AMR. #
# ==================================================================== #
#' AMR plots with `ggplot2`
#'
#' Use these functions to create bar plots for antimicrobial resistance analysis. All functions rely on internal [ggplot2][ggplot2::ggplot()] functions.
#' @inheritSection lifecycle Maturing lifecycle
#' @param data a [`data.frame`] with column(s) of class [`rsi`] (see [as.rsi()])
#' @param position position adjustment of bars, either `"fill"`, `"stack"` or `"dodge"`
#' @param x variable to show on x axis, either `"antibiotic"` (default) or `"interpretation"` or a grouping variable
#' @param fill variable to categorise using the plots legend, either `"antibiotic"` (default) or `"interpretation"` or a grouping variable
#' @param breaks numeric vector of positions
#' @param limits numeric vector of length two providing limits of the scale, use `NA` to refer to the existing minimum or maximum
#' @param facet variable to split plots by, either `"interpretation"` (default) or `"antibiotic"` or a grouping variable
#' @inheritParams proportion
#' @param nrow (when using `facet`) number of rows
#' @param colours a named vector with colours for the bars. The names must be one or more of: S, SI, I, IR, R or be `FALSE` to use default [ggplot2][[ggplot2::ggplot()] colours.
#' @param datalabels show datalabels using [labels_rsi_count()]
#' @param datalabels.size size of the datalabels
#' @param datalabels.colour colour of the datalabels
#' @param title text to show as title of the plot
#' @param subtitle text to show as subtitle of the plot
#' @param caption text to show as caption of the plot
#' @param x.title text to show as x axis description
#' @param y.title text to show as y axis description
#' @param ... other parameters passed on to [geom_rsi()]
#' @details At default, the names of antibiotics will be shown on the plots using [ab_name()]. This can be set with the `translate_ab` parameter. See [count_df()].
#'
#' ## The functions
#' [geom_rsi()] will take any variable from the data that has an [`rsi`] class (created with [as.rsi()]) using [rsi_df()] and will plot bars with the percentage R, I and S. The default behaviour is to have the bars stacked and to have the different antibiotics on the x axis.
#'
#' [facet_rsi()] creates 2d plots (at default based on S/I/R) using [ggplot2::facet_wrap()].
#'
#' [scale_y_percent()] transforms the y axis to a 0 to 100% range using [ggplot2::scale_continuous()].
#'
#' [scale_rsi_colours()] sets colours to the bars: pastel blue for S, pastel turquoise for I and pastel red for R, using [ggplot2::scale_brewer()].
#'
#' [theme_rsi()] is a [ggplot2 theme][[ggplot2::theme()] with minimal distraction.
#'
#' [labels_rsi_count()] print datalabels on the bars with percentage and amount of isolates using [ggplot2::geom_text()]
#'
#' [ggplot_rsi()] is a wrapper around all above functions that uses data as first input. This makes it possible to use this function after a pipe (`%>%`). See Examples.
#' @rdname ggplot_rsi
#' @export
#' @inheritSection AMR Read more on our website!
#' @examples
#' library(dplyr)
#' library(ggplot2)
#'
#' # get antimicrobial results for drugs against a UTI:
#' ggplot(example_isolates %>% select(AMX, NIT, FOS, TMP, CIP)) +
#' geom_rsi()
#'
#' # prettify the plot using some additional functions:
#' df <- example_isolates %>% select(AMX, NIT, FOS, TMP, CIP)
#' ggplot(df) +
#' geom_rsi() +
#' scale_y_percent() +
#' scale_rsi_colours() +
#' labels_rsi_count() +
#' theme_rsi()
#'
#' # or better yet, simplify this using the wrapper function - a single command:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi()
#'
#' # get only proportions and no counts:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi(datalabels = FALSE)
#'
#' # add other ggplot2 parameters as you like:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi(width = 0.5,
#' colour = "black",
#' size = 1,
#' linetype = 2,
#' alpha = 0.25)
#'
#' example_isolates %>%
#' select(AMX) %>%
#' ggplot_rsi(colours = c(SI = "yellow"))
#'
#' \dontrun{
#'
#' # resistance of ciprofloxacine per age group
#' example_isolates %>%
#' mutate(first_isolate = first_isolate(.)) %>%
#' filter(first_isolate == TRUE,
#' mo == as.mo("E. coli")) %>%
#' # `age_group` is also a function of this package:
#' group_by(age_group = age_groups(age)) %>%
#' select(age_group,
#' CIP) %>%
#' ggplot_rsi(x = "age_group")
#'
#' # for colourblind mode, use divergent colours from the viridis package:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi() + scale_fill_viridis_d()
#' # a shorter version which also adjusts data label colours:
#' example_isolates %>%
#' select(AMX, NIT, FOS, TMP, CIP) %>%
#' ggplot_rsi(colours = FALSE)
#'
#'
#' # it also supports groups (don't forget to use the group var on `x` or `facet`):
#' example_isolates %>%
#' select(hospital_id, AMX, NIT, FOS, TMP, CIP) %>%
#' group_by(hospital_id) %>%
#' ggplot_rsi(x = "hospital_id",
#' facet = "antibiotic",
#' nrow = 1,
#' title = "AMR of Anti-UTI Drugs Per Hospital",
#' x.title = "Hospital",
#' datalabels = FALSE)
#'
#' # genuine analysis: check 3 most prevalent microorganisms
#' example_isolates %>%
#' # create new bacterial ID's, with all CoNS under the same group (Becker et al.)
#' mutate(mo = as.mo(mo, Becker = TRUE)) %>%
#' # filter on top three bacterial ID's
#' filter(mo %in% top_freq(freq(.$mo), 3)) %>%
#' # filter on first isolates
#' filter_first_isolate() %>%
#' # get short MO names (like "E. coli")
#' mutate(bug = mo_shortname(mo, Becker = TRUE)) %>%
#' # select this short name and some antiseptic drugs
#' select(bug, CXM, GEN, CIP) %>%
#' # group by MO
#' group_by(bug) %>%
#' # plot the thing, putting MOs on the facet
#' ggplot_rsi(x = "antibiotic",
#' facet = "bug",
#' translate_ab = FALSE,
#' nrow = 1,
#' title = "AMR of Top Three Microorganisms In Blood Culture Isolates",
#' subtitle = expression(paste("Only First Isolates, CoNS grouped according to Becker ",
#' italic("et al."), " (2014)")),
#' x.title = "Antibiotic (EARS-Net code)")
#' }
ggplot_rsi <- function(data,
position = NULL,
x = "antibiotic",
fill = "interpretation",
# params = list(),
facet = NULL,
breaks = seq(0, 1, 0.1),
limits = NULL,
translate_ab = "name",
combine_SI = TRUE,
combine_IR = FALSE,
language = get_locale(),
nrow = NULL,
colours = c(S = "#61a8ff",
SI = "#61a8ff",
I = "#61f7ff",
IR = "#ff6961",
R = "#ff6961"),
datalabels = TRUE,
datalabels.size = 2.5,
datalabels.colour = "gray15",
title = NULL,
subtitle = NULL,
caption = NULL,
x.title = "Antimicrobial",
y.title = "Proportion",
...) {
stopifnot_installed_package("ggplot2")
x <- x[1]
facet <- facet[1]
# we work with aes_string later on
x_deparse <- deparse(substitute(x))
if (x_deparse != "x") {
x <- x_deparse
}
if (x %like% '".*"') {
x <- substr(x, 2, nchar(x) - 1)
}
facet_deparse <- deparse(substitute(facet))
if (facet_deparse != "facet") {
facet <- facet_deparse
}
if (facet %like% '".*"') {
facet <- substr(facet, 2, nchar(facet) - 1)
}
if (facet %in% c("NULL", "")) {
facet <- NULL
}
if (is.null(position)) {
position <- "fill"
}
p <- ggplot2::ggplot(data = data) +
geom_rsi(position = position, x = x, fill = fill, translate_ab = translate_ab,
combine_SI = combine_SI, combine_IR = combine_IR, ...) +
theme_rsi()
if (fill == "interpretation") {
# set RSI colours
if (isFALSE(colours) & missing(datalabels.colour)) {
# set datalabel colour to middle gray
datalabels.colour <- "gray50"
}
p <- p + scale_rsi_colours(colours = colours)
}
if (identical(position, "fill")) {
# proportions, so use y scale with percentage
p <- p + scale_y_percent(breaks = breaks, limits = limits)
}
if (datalabels == TRUE) {
p <- p + labels_rsi_count(position = position,
x = x,
translate_ab = translate_ab,
combine_SI = combine_SI,
combine_IR = combine_IR,
datalabels.size = datalabels.size,
datalabels.colour = datalabels.colour)
}
if (!is.null(facet)) {
p <- p + facet_rsi(facet = facet, nrow = nrow)
}
p <- p + ggplot2::labs(title = title,
subtitle = subtitle,
caption = caption,
x = x.title,
y = y.title)
p
}
#' @rdname ggplot_rsi
#' @export
geom_rsi <- function(position = NULL,
x = c("antibiotic", "interpretation"),
fill = "interpretation",
translate_ab = "name",
language = get_locale(),
combine_SI = TRUE,
combine_IR = FALSE,
...) {
stopifnot_installed_package("ggplot2")
if (is.data.frame(position)) {
stop("`position` is invalid. Did you accidentally use '%>%' instead of '+'?", call. = FALSE)
}
y <- "value"
if (missing(position) | is.null(position)) {
position <- "fill"
}
if (identical(position, "fill")) {
position <- ggplot2::position_fill(vjust = 0.5, reverse = TRUE)
}
x <- x[1]
# we work with aes_string later on
x_deparse <- deparse(substitute(x))
if (x_deparse != "x") {
x <- x_deparse
}
if (x %like% '".*"') {
x <- substr(x, 2, nchar(x) - 1)
}
if (tolower(x) %in% tolower(c("ab", "abx", "antibiotics"))) {
x <- "antibiotic"
} else if (tolower(x) %in% tolower(c("SIR", "RSI", "interpretations", "result"))) {
x <- "interpretation"
}
ggplot2::layer(geom = "bar", stat = "identity", position = position,
mapping = ggplot2::aes_string(x = x, y = y, fill = fill),
params = list(...), data = function(x) {
rsi_df(data = x,
translate_ab = translate_ab,
language = language,
combine_SI = combine_SI,
combine_IR = combine_IR)
})
}
#' @rdname ggplot_rsi
#' @export
facet_rsi <- function(facet = c("interpretation", "antibiotic"), nrow = NULL) {
stopifnot_installed_package("ggplot2")
facet <- facet[1]
# we work with aes_string later on
facet_deparse <- deparse(substitute(facet))
if (facet_deparse != "facet") {
facet <- facet_deparse
}
if (facet %like% '".*"') {
facet <- substr(facet, 2, nchar(facet) - 1)
}
if (tolower(facet) %in% tolower(c("SIR", "RSI", "interpretations", "result"))) {
facet <- "interpretation"
} else if (tolower(facet) %in% tolower(c("ab", "abx", "antibiotics"))) {
facet <- "antibiotic"
}
ggplot2::facet_wrap(facets = facet, scales = "free_x", nrow = nrow)
}
#' @rdname ggplot_rsi
#' @importFrom cleaner percentage
#' @export
scale_y_percent <- function(breaks = seq(0, 1, 0.1), limits = NULL) {
stopifnot_installed_package("ggplot2")
if (all(breaks[breaks != 0] > 1)) {
breaks <- breaks / 100
}
ggplot2::scale_y_continuous(breaks = breaks,
labels = percentage(breaks),
limits = limits)
}
#' @rdname ggplot_rsi
#' @export
scale_rsi_colours <- function(colours = c(S = "#61a8ff",
SI = "#61a8ff",
I = "#61f7ff",
IR = "#ff6961",
R = "#ff6961")) {
stopifnot_installed_package("ggplot2")
# previous colour: palette = "RdYlGn"
# previous colours: values = c("#b22222", "#ae9c20", "#7cfc00")
if (!identical(colours, FALSE)) {
original_cols <- c(S = "#61a8ff",
SI = "#61a8ff",
I = "#61f7ff",
IR = "#ff6961",
R = "#ff6961")
colours <- replace(original_cols, names(colours), colours)
ggplot2::scale_fill_manual(values = colours)
}
}
#' @rdname ggplot_rsi
#' @export
theme_rsi <- function() {
stopifnot_installed_package("ggplot2")
ggplot2::theme_minimal(base_size = 10) +
ggplot2::theme(panel.grid.major.x = ggplot2::element_blank(),
panel.grid.minor = ggplot2::element_blank(),
panel.grid.major.y = ggplot2::element_line(colour = "grey75"),
# center title and subtitle
plot.title = ggplot2::element_text(hjust = 0.5),
plot.subtitle = ggplot2::element_text(hjust = 0.5))
}
#' @rdname ggplot_rsi
#' @importFrom dplyr mutate %>% group_by_at
#' @importFrom cleaner percentage
#' @export
labels_rsi_count <- function(position = NULL,
x = "antibiotic",
translate_ab = "name",
combine_SI = TRUE,
combine_IR = FALSE,
datalabels.size = 3,
datalabels.colour = "gray15") {
stopifnot_installed_package("ggplot2")
if (is.null(position)) {
position <- "fill"
}
if (identical(position, "fill")) {
position <- ggplot2::position_fill(vjust = 0.5, reverse = TRUE)
}
x_name <- x
ggplot2::geom_text(mapping = ggplot2::aes_string(label = "lbl",
x = x,
y = "value"),
position = position,
inherit.aes = FALSE,
size = datalabels.size,
colour = datalabels.colour,
lineheight = 0.75,
data = function(x) {
rsi_df(data = x,
translate_ab = translate_ab,
combine_SI = combine_SI,
combine_IR = combine_IR) %>%
group_by_at(x_name) %>%
mutate(lbl = paste0("n=", isolates))
})
}
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