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538 lines
20 KiB
R
Executable File
538 lines
20 KiB
R
Executable File
# ==================================================================== #
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# TITLE #
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# Antimicrobial Resistance (AMR) Analysis #
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# #
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# SOURCE #
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# https://gitlab.com/msberends/AMR #
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# #
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# LICENCE #
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# (c) 2018-2020 Berends MS, Luz CF et al. #
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# #
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# This R package is free software; you can freely use and distribute #
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# it for both personal and commercial purposes under the terms of the #
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# GNU General Public License version 2.0 (GNU GPL-2), as published by #
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# the Free Software Foundation. #
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# #
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# We created this package for both routine data analysis and academic #
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# research and it was publicly released in the hope that it will be #
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# useful, but it comes WITHOUT ANY WARRANTY OR LIABILITY. #
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# Visit our website for more info: https://msberends.gitlab.io/AMR. #
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# ==================================================================== #
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#' Class 'rsi'
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#'
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#' Interpret MIC values and disk diffusion diameters according to EUCAST or CLSI, or clean up existing R/SI values. This transforms the input to a new class [`rsi`], which is an ordered factor with levels `S < I < R`. Invalid antimicrobial interpretations will be translated as `NA` with a warning.
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#' @inheritSection lifecycle Stable lifecycle
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#' @rdname as.rsi
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#' @param x vector of values (for class [`mic`]: an MIC value in mg/L, for class [`disk`]: a disk diffusion radius in millimetres)
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#' @param mo any (vector of) text that can be coerced to a valid microorganism code with [as.mo()]
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#' @param ab any (vector of) text that can be coerced to a valid antimicrobial code with [as.ab()]
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#' @inheritParams first_isolate
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#' @param guideline defaults to the latest included EUCAST guideline, run `unique(rsi_translation$guideline)` for all options
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#' @param threshold maximum fraction of invalid antimicrobial interpretations of `x`, please see *Examples*
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#' @param ... parameters passed on to methods
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#' @details Run `unique(rsi_translation$guideline)` for a list of all supported guidelines. The repository of this package contains [this machine readable version](https://gitlab.com/msberends/AMR/blob/master/data-raw/rsi_translation.txt) of these guidelines.
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#'
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#' These guidelines are machine readable, since [](https://gitlab.com/msberends/AMR/blob/master/data-raw/rsi_translation.txt).
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#'
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#' After using [as.rsi()], you can use [eucast_rules()] to (1) apply inferred susceptibility and resistance based on results of other antimicrobials and (2) apply intrinsic resistance based on taxonomic properties of a microorganism.
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#'
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#' The function [is.rsi.eligible()] returns `TRUE` when a columns contains at most 5% invalid antimicrobial interpretations (not S and/or I and/or R), and `FALSE` otherwise. The threshold of 5% can be set with the `threshold` parameter.
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#' @section Interpretation of R and S/I:
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#' In 2019, the European Committee on Antimicrobial Susceptibility Testing (EUCAST) has decided to change the definitions of susceptibility testing categories R and S/I as shown below (<http://www.eucast.org/newsiandr/>).
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#'
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#' - **R = Resistant**\cr
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#' A microorganism is categorised as *Resistant* when there is a high likelihood of therapeutic failure even when there is increased exposure. Exposure is a function of how the mode of administration, dose, dosing interval, infusion time, as well as distribution and excretion of the antimicrobial agent will influence the infecting organism at the site of infection.
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#' - **S = Susceptible**\cr
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#' A microorganism is categorised as *Susceptible, standard dosing regimen*, when there is a high likelihood of therapeutic success using a standard dosing regimen of the agent.
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#' - **I = Increased exposure, but still susceptible**\cr
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#' A microorganism is categorised as *Susceptible, Increased exposure* when there is a high likelihood of therapeutic success because exposure to the agent is increased by adjusting the dosing regimen or by its concentration at the site of infection.
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#'
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#' This AMR package honours this new insight. Use [susceptibility()] (equal to [proportion_SI()]) to determine antimicrobial susceptibility and [count_susceptible()] (equal to [count_SI()]) to count susceptible isolates.
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#' @return Ordered factor with new class [`rsi`]
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#' @aliases rsi
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#' @export
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#' @importFrom dplyr %>% desc arrange filter
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#' @seealso [as.mic()]
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#' @inheritSection AMR Read more on our website!
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#' @examples
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#' # For INTERPRETING disk diffusion and MIC values -----------------------
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#'
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#' # single values
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#' as.rsi(x = as.mic(2),
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#' mo = as.mo("S. pneumoniae"),
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#' ab = "AMP",
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#' guideline = "EUCAST")
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#'
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#' as.rsi(x = as.disk(18),
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#' mo = "Strep pneu", # `mo` will be coerced with as.mo()
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#' ab = "ampicillin", # and `ab` with as.ab()
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#' guideline = "EUCAST")
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#'
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#' # a whole data set, even with combined MIC values and disk zones
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#' df <- data.frame(microorganism = "E. coli",
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#' AMP = as.mic(8),
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#' CIP = as.mic(0.256),
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#' GEN = as.disk(18),
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#' TOB = as.disk(16))
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#' as.rsi(df)
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#'
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#'
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#' # For CLEANING existing R/SI values ------------------------------------
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#'
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#' as.rsi(c("S", "I", "R", "A", "B", "C"))
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#' as.rsi("<= 0.002; S") # will return "S"
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#'
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#' rsi_data <- as.rsi(c(rep("S", 474), rep("I", 36), rep("R", 370)))
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#' is.rsi(rsi_data)
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#' plot(rsi_data) # for percentages
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#' barplot(rsi_data) # for frequencies
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#' freq(rsi_data) # frequency table with informative header
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#'
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#' library(dplyr)
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#' example_isolates %>%
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#' mutate_at(vars(PEN:RIF), as.rsi)
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#'
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#' # fastest way to transform all columns with already valid AMR results to class `rsi`:
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#' example_isolates %>%
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#' mutate_if(is.rsi.eligible, as.rsi)
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#'
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#' # note: from dplyr 1.0.0 on, this will be:
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#' # example_isolates %>%
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#' # mutate(across(is.rsi.eligible, as.rsi))
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#'
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#' # default threshold of `is.rsi.eligible` is 5%.
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#' is.rsi.eligible(WHONET$`First name`) # fails, >80% is invalid
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#' is.rsi.eligible(WHONET$`First name`, threshold = 0.99) # succeeds
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as.rsi <- function(x, ...) {
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UseMethod("as.rsi")
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}
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#' @export
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as.rsi.default <- function(x, ...) {
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if (is.rsi(x)) {
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x
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} else if (identical(levels(x), c("S", "I", "R"))) {
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structure(x, class = c("rsi", "ordered", "factor"))
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} else if (identical(class(x), "integer") & all(x %in% c(1:3, NA))) {
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x[x == 1] <- "S"
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x[x == 2] <- "I"
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x[x == 3] <- "R"
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structure(.Data = factor(x, levels = c("S", "I", "R"), ordered = TRUE),
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class = c("rsi", "ordered", "factor"))
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} else {
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x <- x %>% unlist()
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x.bak <- x
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na_before <- x[is.na(x) | x == ""] %>% length()
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# remove all spaces
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x <- gsub(" +", "", x)
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# remove all MIC-like values: numbers, operators and periods
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x <- gsub("[0-9.,;:<=>]+", "", x)
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# remove everything between brackets, and 'high' and 'low'
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x <- gsub("([(].*[)])", "", x)
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x <- gsub("(high|low)", "", x, ignore.case = TRUE)
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# disallow more than 3 characters
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x[nchar(x) > 3] <- NA
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# set to capitals
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x <- toupper(x)
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# remove all invalid characters
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x <- gsub("[^RSI]+", "", x)
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# in cases of "S;S" keep S, but in case of "S;I" make it NA
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x <- gsub("^S+$", "S", x)
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x <- gsub("^I+$", "I", x)
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x <- gsub("^R+$", "R", x)
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x[!x %in% c("S", "I", "R")] <- NA
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na_after <- x[is.na(x) | x == ""] %>% length()
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if (!isFALSE(list(...)$warn)) { # so as.rsi(..., warn = FALSE) will never throw a warning
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if (na_before != na_after) {
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list_missing <- x.bak[is.na(x) & !is.na(x.bak) & x.bak != ""] %>%
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unique() %>%
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sort()
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list_missing <- paste0('"', list_missing, '"', collapse = ", ")
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warning(na_after - na_before, " results truncated (",
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round(((na_after - na_before) / length(x)) * 100),
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"%) that were invalid antimicrobial interpretations: ",
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list_missing, call. = FALSE)
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}
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}
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structure(.Data = factor(x, levels = c("S", "I", "R"), ordered = TRUE),
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class = c("rsi", "ordered", "factor"))
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}
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}
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input_resembles_mic <- function(x) {
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mic <- x %>%
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gsub("[^0-9.,]+", "", .) %>%
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unique()
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mic_valid <- suppressWarnings(as.mic(mic))
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result <- sum(!is.na(mic_valid)) / length(mic)
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if (is.na(result)) {
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0
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} else {
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result
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}
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}
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#' @rdname as.rsi
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#' @importFrom dplyr case_when
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#' @export
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as.rsi.mic <- function(x, mo, ab, guideline = "EUCAST", ...) {
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exec_as.rsi(method = "mic",
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x = x,
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mo = mo,
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ab = ab,
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guideline = guideline)
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}
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#' @rdname as.rsi
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#' @export
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as.rsi.disk <- function(x, mo, ab, guideline = "EUCAST", ...) {
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exec_as.rsi(method = "disk",
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x = x,
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mo = mo,
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ab = ab,
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guideline = guideline)
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}
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get_guideline <- function(guideline) {
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guideline_param <- toupper(guideline)
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if (guideline_param %in% c("CLSI", "EUCAST")) {
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guideline_param <- rsi_translation %>%
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filter(guideline %like% guideline_param) %>%
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pull(guideline) %>%
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sort() %>%
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rev() %>%
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.[1]
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}
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if (!guideline_param %in% rsi_translation$guideline) {
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stop(paste0("invalid guideline: '", guideline,
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"'.\nValid guidelines are: ", paste0("'", rev(sort(unique(rsi_translation$guideline))), "'", collapse = ", ")),
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call. = FALSE)
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}
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guideline_param
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}
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exec_as.rsi <- function(method, x, mo, ab, guideline) {
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if (method == "mic") {
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x <- as.double(as.mic(x)) # when as.rsi.mic is called directly
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method_param <- "MIC"
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} else if (method == "disk") {
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x <- as.double(as.disk(x)) # when as.rsi.disk is called directly
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method_param <- "DISK"
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}
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mo <- as.mo(mo)
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ab <- as.ab(ab)
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mo_genus <- as.mo(mo_genus(mo))
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mo_family <- as.mo(mo_family(mo))
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mo_order <- as.mo(mo_order(mo))
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mo_becker <- as.mo(mo, Becker = TRUE)
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mo_lancefield <- as.mo(mo, Lancefield = TRUE)
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mo_other <- as.mo("other")
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guideline_coerced <- get_guideline(guideline)
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if (guideline_coerced != guideline) {
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message(blue(paste0("Note: Using guideline ", bold(guideline_coerced), " as input for `guideline`.")))
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}
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new_rsi <- rep(NA_character_, length(x))
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trans <- rsi_translation %>%
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filter(guideline == guideline_coerced & method == method_param) %>%
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mutate(lookup = paste(mo, ab))
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lookup_mo <- paste(mo, ab)
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lookup_genus <- paste(mo_genus, ab)
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lookup_family <- paste(mo_family, ab)
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lookup_order <- paste(mo_order, ab)
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lookup_becker <- paste(mo_becker, ab)
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lookup_lancefield <- paste(mo_lancefield, ab)
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lookup_other <- paste(mo_other, ab)
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for (i in seq_len(length(x))) {
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get_record <- trans %>%
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filter(lookup %in% c(lookup_mo[i],
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lookup_genus[i],
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lookup_family[i],
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lookup_order[i],
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lookup_becker[i],
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lookup_lancefield[i],
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lookup_other[i])) %>%
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# be as specific as possible (i.e. prefer species over genus):
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arrange(desc(nchar(mo))) %>%
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.[1L, ]
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if (NROW(get_record) > 0) {
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if (is.na(x[i])) {
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new_rsi[i] <- NA_character_
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} else if (method == "mic") {
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new_rsi[i] <- case_when(isTRUE(x[i] <= get_record$breakpoint_S) ~ "S",
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isTRUE(x[i] >= get_record$breakpoint_R) ~ "R",
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!is.na(get_record$breakpoint_S) & !is.na(get_record$breakpoint_R) ~ "I",
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TRUE ~ NA_character_)
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} else if (method == "disk") {
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new_rsi[i] <- case_when(isTRUE(x[i] >= get_record$breakpoint_S) ~ "S",
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isTRUE(x[i] <= get_record$breakpoint_R) ~ "R",
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!is.na(get_record$breakpoint_S) & !is.na(get_record$breakpoint_R) ~ "I",
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TRUE ~ NA_character_)
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}
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}
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}
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structure(.Data = factor(new_rsi, levels = c("S", "I", "R"), ordered = TRUE),
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class = c("rsi", "ordered", "factor"))
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}
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#' @rdname as.rsi
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#' @importFrom crayon red blue bold
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#' @export
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as.rsi.data.frame <- function(x, col_mo = NULL, guideline = "EUCAST", ...) {
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x <- x
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ab_cols <- colnames(x)[sapply(x, function(y) is.mic(y) | is.disk(y))]
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if (length(ab_cols) == 0) {
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stop("No columns with MIC values or disk zones found in this data set. Use as.mic or as.disk to transform antimicrobial columns.", call. = FALSE)
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}
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# try to find columns based on type
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# -- mo
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if (is.null(col_mo)) {
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col_mo <- search_type_in_df(x = x, type = "mo")
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}
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if (is.null(col_mo)) {
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stop("`col_mo` must be set.", call. = FALSE)
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}
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guideline_coerced <- get_guideline(guideline)
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if (guideline_coerced != guideline) {
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message(blue(paste0("Note: Using guideline ", bold(guideline_coerced), " as input for `guideline`.")))
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}
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# transform all MICs
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ab_cols <- colnames(x)[sapply(x, is.mic)]
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if (length(ab_cols) > 0) {
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for (i in seq_len(length(ab_cols))) {
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ab_col_coerced <- suppressWarnings(as.ab(ab_cols[i]))
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if (is.na(ab_col_coerced)) {
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message(red(paste0("Unknown drug: `", bold(ab_cols[i]), "`. Rename this column to a drug name or code, and check the output with as.ab().")))
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next
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}
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message(blue(paste0("Interpreting MIC values of column `", bold(ab_cols[i]), "` (",
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ifelse(ab_col_coerced != ab_cols[i], paste0(ab_col_coerced, ", "), ""),
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ab_name(ab_col_coerced, tolower = TRUE), ")...")),
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appendLF = FALSE)
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x[, ab_cols[i]] <- exec_as.rsi(method = "mic",
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x = x %>% pull(ab_cols[i]),
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mo = x %>% pull(col_mo),
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ab = ab_col_coerced,
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guideline = guideline_coerced)
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message(blue(" OK."))
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}
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}
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# transform all disks
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ab_cols <- colnames(x)[sapply(x, is.disk)]
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if (length(ab_cols) > 0) {
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for (i in seq_len(length(ab_cols))) {
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ab_col_coerced <- suppressWarnings(as.ab(ab_cols[i]))
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if (is.na(ab_col_coerced)) {
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message(red(paste0("Unknown drug: `", bold(ab_cols[i]), "`. Rename this column to a drug name or code, and check the output with as.ab().")))
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next
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}
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message(blue(paste0("Interpreting disk zones of column `", bold(ab_cols[i]), "` (",
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ifelse(ab_col_coerced != ab_cols[i], paste0(ab_col_coerced, ", "), ""),
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ab_name(ab_col_coerced, tolower = TRUE), ")...")),
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appendLF = FALSE)
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x[, ab_cols[i]] <- exec_as.rsi(method = "disk",
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x = x %>% pull(ab_cols[i]),
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mo = x %>% pull(col_mo),
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ab = ab_col_coerced,
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guideline = guideline_coerced)
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message(blue(" OK."))
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}
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}
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x
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}
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#' @rdname as.rsi
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#' @export
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is.rsi <- function(x) {
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inherits(x, "rsi")
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}
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#' @rdname as.rsi
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#' @export
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is.rsi.eligible <- function(x, threshold = 0.05) {
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if (NCOL(x) > 1) {
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stop("`x` must be a one-dimensional vector.")
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}
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if (any(c("logical",
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"numeric",
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"integer",
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"mo",
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"Date",
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"POSIXct",
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"rsi",
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"raw",
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"hms")
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%in% class(x))) {
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# no transformation needed
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FALSE
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} else {
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x <- x[!is.na(x) & !is.null(x) & !identical(x, "")]
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if (length(x) == 0) {
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return(FALSE)
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}
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checked <- suppressWarnings(as.rsi(x))
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outcome <- sum(is.na(checked)) / length(x)
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outcome <= threshold
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}
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}
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#' @exportMethod print.rsi
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#' @export
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#' @importFrom dplyr %>%
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#' @noRd
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print.rsi <- function(x, ...) {
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cat("Class 'rsi'\n")
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print(as.character(x), quote = FALSE)
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}
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#' @exportMethod droplevels.rsi
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#' @export
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#' @noRd
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droplevels.rsi <- function(x, exclude = if (anyNA(levels(x))) NULL else NA, ...) {
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x <- droplevels.factor(x, exclude = exclude, ...)
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class(x) <- c("rsi", "ordered", "factor")
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x
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}
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#' @exportMethod summary.rsi
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#' @export
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#' @noRd
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summary.rsi <- function(object, ...) {
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x <- object
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c(
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"Class" = "rsi",
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"<NA>" = sum(is.na(x)),
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"Sum S" = sum(x == "S", na.rm = TRUE),
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"Sum IR" = sum(x %in% c("I", "R"), na.rm = TRUE),
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"-Sum R" = sum(x == "R", na.rm = TRUE),
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"-Sum I" = sum(x == "I", na.rm = TRUE)
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)
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}
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#' @exportMethod plot.rsi
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#' @export
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#' @importFrom dplyr %>% group_by summarise filter mutate if_else n_distinct
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#' @importFrom graphics plot text
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#' @noRd
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plot.rsi <- function(x,
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lwd = 2,
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ylim = NULL,
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ylab = "Percentage",
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xlab = "Antimicrobial Interpretation",
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main = paste("Susceptibility Analysis of", deparse(substitute(x))),
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axes = FALSE,
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...) {
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suppressWarnings(
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data <- data.frame(x = x,
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y = 1,
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stringsAsFactors = TRUE) %>%
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group_by(x) %>%
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summarise(n = sum(y)) %>%
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filter(!is.na(x)) %>%
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mutate(s = round((n / sum(n)) * 100, 1))
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)
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if (!"S" %in% data$x) {
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data <- rbind(data, data.frame(x = "S", n = 0, s = 0))
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}
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if (!"I" %in% data$x) {
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data <- rbind(data, data.frame(x = "I", n = 0, s = 0))
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}
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if (!"R" %in% data$x) {
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data <- rbind(data, data.frame(x = "R", n = 0, s = 0))
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}
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data$x <- factor(data$x, levels = c("S", "I", "R"), ordered = TRUE)
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ymax <- if_else(max(data$s) > 95, 105, 100)
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plot(x = data$x,
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y = data$s,
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lwd = lwd,
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ylim = c(0, ymax),
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ylab = ylab,
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xlab = xlab,
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main = main,
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axes = axes,
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...)
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# x axis
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axis(side = 1, at = 1:n_distinct(data$x), labels = levels(data$x), lwd = 0)
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# y axis, 0-100%
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axis(side = 2, at = seq(0, 100, 5))
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text(x = data$x,
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y = data$s + 4,
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labels = paste0(data$s, "% (n = ", data$n, ")"))
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}
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#' @exportMethod barplot.rsi
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#' @export
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#' @importFrom dplyr %>% group_by summarise
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#' @importFrom graphics barplot axis par
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#' @noRd
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barplot.rsi <- function(height,
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col = c("green3", "orange2", "red3"),
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xlab = ifelse(beside, "Antimicrobial Interpretation", ""),
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main = paste("Susceptibility Analysis of", deparse(substitute(height))),
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ylab = "Frequency",
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beside = TRUE,
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axes = beside,
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...) {
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if (axes == TRUE) {
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par(mar = c(5, 4, 4, 2) + 0.1)
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} else {
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par(mar = c(2, 4, 4, 2) + 0.1)
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}
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barplot(as.matrix(table(height)),
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col = col,
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xlab = xlab,
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main = main,
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ylab = ylab,
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beside = beside,
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axes = FALSE,
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...)
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# y axis, 0-100%
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axis(side = 2, at = seq(0, max(table(height)) + max(table(height)) * 1.1, by = 25))
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if (axes == TRUE && beside == TRUE) {
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axis(side = 1, labels = levels(height), at = c(1, 2, 3) + 0.5, lwd = 0)
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}
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}
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#' @importFrom pillar type_sum
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#' @export
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type_sum.rsi <- function(x) {
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"rsi"
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}
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#' @importFrom pillar pillar_shaft
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#' @importFrom crayon bgGreen bgYellow bgRed black white
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#' @export
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pillar_shaft.rsi <- function(x, ...) {
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out <- trimws(format(x))
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out[is.na(x)] <- pillar::style_subtle(" NA")
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out[x == "S"] <- bgGreen(white(" S "))
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out[x == "I"] <- bgYellow(black(" I "))
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out[x == "R"] <- bgRed(white(" R "))
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pillar::new_pillar_shaft_simple(out, align = "left", width = 3)
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}
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