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# RNAseq pipeline
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Snippets for the RNAseq pipeline:
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https://docs.google.com/document/d/1lM1wlz950Q8H6Oi7CWW28FO2tGv5nXYtW0wp8vEUizA
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## Generate Raw Counts using FeatureCounts/HTSeqCounts
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## Load package Subread (packge within which FeatureCounts is present)
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+ ml Subread/1.6.4-foss-2015b FeatureCounts
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## Features used in FeatureCounts :
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## -s : reversely stranded
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## -T : number of threads used
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## -a : reference genome
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## -o : output file
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+ featureCounts \
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+ --a "path to reference genome" \
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+ --T 6 \
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+ --o "path to output file" \
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+ --s 2
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